Loss of interleukin-1 beta is not protective in the lupus-prone NZM2328 mouse model. Loss of interleukin-1 beta is not protective in the lupus-prone NZM2328 mouse model

RNA-sequencing analysis identified upregulation of TNF and IL-17 signaling pathways specifically in females lacking IL-1β. Increases in these signaling pathways are also found in female patients with lupus nephritis, suggesting clinical relevance for upregulation of these pathways. Overall design: To better understand the impact of IL-1β deletion on lupus kidneys, genome-wide transcriptome analysis using RNA-sequencing (RNA-seq) was performed and transcriptional differences in the kidneys of male and female NZM and NZM-Il1b-/- mice were examined. Kidney RNA was isolated via Zymo Direct-zol RNA isolation kit and libraries were generated using standard poly-A prep kits (New England Biolabs) with the assistance of the U-M Advanced Genomics Core.

RNA测序（RNA-sequencing）分析显示，在缺失白细胞介素1β（Interleukin 1β, IL-1β）的雌性小鼠中，肿瘤坏死因子（Tumor Necrosis Factor, TNF）与白细胞介素17（Interleukin 17, IL-17）信号通路出现特异性上调。此类信号通路的上调同样见于狼疮肾炎（Lupus Nephritis）女性患者，提示上述通路的上调具有临床相关性。

整体实验设计：为深入解析白细胞介素1β（IL-1β）基因敲除对狼疮性肾脏的影响，本研究采用RNA测序（RNA-sequencing, 简称RNA-seq）开展全基因组转录组分析，对雄性、雌性NZM小鼠及IL-1β基因敲除型NZM（NZM-Il1b-/-）小鼠的肾脏组织转录差异进行检测。肾脏总RNA通过Zymo Direct-zol RNA提取试剂盒完成分离，文库构建采用标准polyA建库试剂盒（New England Biolabs，新英格兰生物实验室），并由密歇根大学（University of Michigan, U-M）高级基因组学核心实验室协助完成。