Integrating single-cell Mendelian Randomization, bulk RNA sequencing, and single-cell RNA sequencing data to explore the role of chemokines in Alzheimer's disease

Following the MR, sensitivity, and Steiger test analyses conducted between eQTL-cell pairs and AD, 2,401 eQTL-cell pairs were identified as being associated with AD using the IVW method (Supplementary File 1). Similarly, using the same analytical methods, a total of seven chemokines were identified as having causal associations with AD (Supplementary File 2), followed by 2,149 eQTL-cell pairs with causal relationships to seven chemokines (Supplementary File 3). Based on the integration of these analytical results, 2,149 key eQTL-cell pairs and seven key chemokines were identified.

针对表达数量性状基因座（expression Quantitative Trait Locus, eQTL）-细胞配对与阿尔茨海默病（Alzheimer's Disease, AD）开展孟德尔随机化（Mendelian Randomization, MR）、敏感性分析及施泰格检验（Steiger test）后，本研究通过逆方差加权（Inverse Variance Weighted, IVW）法共鉴定出2401个与AD相关的eQTL-细胞配对（详见补充文件1）。采用相同分析方法，本研究同时鉴定出7种与AD存在因果关联的趋化因子（详见补充文件2），以及2149个与这7种趋化因子存在因果关系的eQTL-细胞配对（详见补充文件3）。基于上述分析结果的整合，最终筛选得到2149个关键eQTL-细胞配对与7种关键趋化因子。