Endothelial Heterogeneity Across Distinct Vascular Beds During Homeostasis and Inflammation Using RiboTag Strategy

Several studies have suggested that endothelial cells which line the blood vessels in each organ significantly differ from each other, but little is known about their distinct and characteristic signatures. We assessed the brain, lung, and heart endothelial translatome using the RiboTag mouse model which allows for the direct isolation of cell-type specific ribosomes and the mRNA undergoing translation. Overall design: Endothelial mRNA translatome profiles of adult Cdh5CreERT2/+; Rpl22HA/+ mice were generated by RNA-Seq using Illumina HiSeq 4000

已有多项研究表明，衬附于各器官血管内壁的内皮细胞（endothelial cells）彼此间存在显著差异，但目前学界对其独特的特征性标记谱仍知之甚少。本研究采用RiboTag小鼠模型（RiboTag mouse model），对脑、肺及心脏的内皮细胞翻译组（translatome）进行了分析，该模型可直接分离细胞类型特异性核糖体及正在进行翻译的信使RNA（mRNA）。整体实验设计：成年Cdh5CreERT2/+; Rpl22HA/+小鼠的内皮细胞mRNA翻译组谱通过Illumina HiSeq 4000平台的RNA测序（RNA-Seq）得以构建。