ExtFig4B_RACE_STOML2_YKT6_R1_LG307.sgd

HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2 or YKT6, and TUBA1B genes, and where indicated, with PR8 PA-X, KSHV SOX or HSV-1 vhs. RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities

HEK293T ishXrn1细胞经多西环素（doxycycline）处理3~4天以诱导Xrn1基因敲低，随后转染携带源自STOML2、YKT6及TUBA1B基因的99 bp插入片段的荧光素酶报告基因载体（luciferase reporters）；在标注的实验组中，共转染PR8 PA-X、卡波西肉瘤相关疱疹病毒（KSHV）SOX及单纯疱疹病毒1型（HSV-1）vhs。提取细胞总RNA后，使用与荧光素酶序列互补的引物开展5'末端快速扩增实验（5' RACE）；纯化所得DNA条带并进行测序，以确认其序列身份。