Transcriptome dynamics and potential roles of Sox6 in the postnatal heart. Mus musculus

To gain an integrated view of the dynamic changes in gene expression during postnatal heart development at the organ level, time-series transcriptome analyses of the postnatal hearts of neonatal through adult mice (P1, P7, P14, P30, and P60) were performed using a newly developed bioinformatics pipeline. Additionally, the role of the transcription factor Sox6 in the postnatal maturation of cardiac muscle was investigated by differential transcriptome analyses between Sox6 knockout (KO) and control hearts. Overall design: Total RNA was extracted from cardiac ventricles of control (MCK-Cre) and Sox6 KO (Sox6loxp/loxp; MCK-Cre) mice at postnatal day 1 (P1), day 7 (P7), day 14 (P14), 1 month (P30), and 2 months (P60). Three biological replicates were prepared for each time point.

为在器官层面全面解析出生后心脏发育过程中基因表达的动态变化规律，本研究采用全新开发的生物信息学（bioinformatics）分析流程，对新生至成年阶段的小鼠出生后心脏（P1、P7、P14、P30及P60）开展了时序转录组（transcriptome）分析。此外，本研究通过对比Sox6基因敲除（knockout, KO）小鼠与对照小鼠的心脏转录组，探究了转录因子（transcription factor）Sox6在心肌出生后成熟过程中的调控作用。总体实验设计：分别于出生后第1天（P1）、第7天（P7）、第14天（P14）、1月龄（P30）及2月龄（P60），从对照（MCK-Cre）与Sox6敲除（Sox6loxp/loxp; MCK-Cre）小鼠的心室组织中提取总RNA，每个时间点设置3次生物学重复（biological replicates）。