Summary of miRNAs sequencing data quality.

ObjectiveThe aim of this study was to explore the differentially expressed miRNAs in the hearts of rats protected from early spontaneous hypertension by blood flow-restricted resistance training and to elucidate the effects of blood flow-restricted resistance training on the expression of these genes.MethodsFour-week-old SHRs and WKY rats were used and randomly divided into five groups: the normal group (WKY), SHR control group (SHR-SED), high-intensity resistance training group (HIRT), medium-intensity resistance training group (MIRT), and blood flow-restricted medium-intensity resistance training group (BFRT). During the experiment, the body weight, cardiac function and hemodynamic parameters of the rats were measured. After training, total RNA was extracted from the left ventricular myocardium of rats in the SHR-SED group and the BFRT group, miRNAs were sequenced, followed by GO enrichment and KEGG pathway analyses, and the differentially expressed miRNAs were subsequently validated via qRT‒PCR.Results1) Hemodynamic tests revealed that the blood pressure of SHRs in the BFRT decreased significantly and that the blood pressure level of SHRs in the BFRT decreased more significantly than that of the simple resistance training groups did (P ConclusionEight weeks of blood flow-restricted medium-intensity resistance training could lower SHR blood pressure, and it might also improve early SHR cardiac function by regulating the expression of miR-224-5p, miR-31a-5p, miR-200b-3p, miR-200a-3p, miR-342-3p, miR-429, miR-1949, miR-1249, and miR-350, with the differential expression of miR-200b-3p being particularly significant.

研究目的
本研究旨在探讨经血流限制性阻力训练保护的早期自发性高血压大鼠心脏中差异表达的微小核糖核酸（microRNAs, 简称miRNAs），并阐明血流限制性阻力训练对这些基因表达的调控作用。

研究方法
选用4周龄的自发性高血压大鼠（Spontaneously Hypertensive Rats, SHRs）与Wistar京都大鼠（WKY rats），随机分为5组：正常对照组（WKY组）、自发性高血压安静对照组（SHR-SED组）、高强度阻力训练组（High-intensity resistance training, HIRT组）、中强度阻力训练组（Medium-intensity resistance training, MIRT组）及血流限制性中强度阻力训练组（Blood flow-restricted medium-intensity resistance training, BFRT组）。实验期间，定期检测大鼠的体重、心功能与血流动力学参数。训练结束后，分别提取SHR-SED组与BFRT组大鼠左心室心肌组织的总RNA，进行miRNAs测序，随后开展基因本体（Gene Ontology, GO）富集分析与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路分析，并通过实时荧光定量PCR（quantitative real-time polymerase chain reaction, qRT-PCR）验证差异表达的miRNAs。

研究结果
1）血流动力学检测结果显示，BFRT组自发性高血压大鼠的血压显著降低，且其降压效果优于单纯阻力训练组（P < 0.05）。

研究结论
8周血流限制性中强度阻力训练可降低自发性高血压大鼠的血压，同时可能通过调控miR-224-5p、miR-31a-5p、miR-200b-3p、miR-200a-3p、miR-342-3p、miR-429、miR-1949、miR-1249及miR-350的表达，改善早期自发性高血压大鼠的心功能，其中miR-200b-3p的差异表达尤为显著。