RNA-seq of Drosophila melanogaster with different genotypes after combinations of pro-longevity interventions

The search for new anti-aging interventions is an issue of great actuality. We report on the success of Drosophila melanogaster lifespan extension under the combined influence of dietary restriction, co-administration of berberine, fucoxanthin, and rapamycin, photodeprivation, and low-temperature conditions up to 185 days in w1118 strain and up to 213 days in long-lived E(z)/w mutants. The trade-off was found between longevity and locomotion. The transcriptome analysis showed an impact of pathways related to epigenetic regulation, lipid metabolism, cellular respiration, nutrient sensing, immune response, and autophagy in the registered effect.

探寻新型抗衰老干预手段是极具现实意义的研究课题。本研究报道了联合应用饮食限制、共同给药小檗碱（berberine）、岩藻黄质（fucoxanthin）与雷帕霉素（rapamycin）、光剥夺以及低温条件下，黑腹果蝇（Drosophila melanogaster）寿命得以延长的研究结果：w1118品系果蝇的最长寿命可达185天，长寿型E(z)/w突变体果蝇的最长寿命可达213天。研究同时发现，寿命延长与运动能力之间存在权衡效应。转录组分析（transcriptome analysis）结果显示，表观遗传调控、脂质代谢、细胞呼吸、营养感知、免疫应答以及自噬相关通路，在本次观测到的寿命延长效应中发挥了重要作用。