RhoBTB1 Reverses Established Arterial Stiffness in Angiotensin II Hypertension

We are investigating of role of RhoBTB1 in vascular smooth muscle cells. Restoring RhoBTB1 expression in mouse aorta reversed the established arterial stiffness but not hypertension caused by angiotensin II (Ang-II). To investigate the underlying mechanism by which RhoBTB1 reversed arterial stiffness, we performed bulk RNA-sequencing using aorta from four groups: control /RhoBTB1 transgenic mice treated with/without Ang-II. Overall design: aortic mRNA profile in vehicle/angiotensin II-treated mice expressing either smooth muscle specific Cre or smooth muscle specific RhoBTB1 transgene.

本研究旨在探究RhoBTB1在血管平滑肌细胞中的功能。在小鼠主动脉中恢复RhoBTB1的表达，可逆转已形成的动脉僵硬度，但无法改善血管紧张素II（angiotensin II, Ang-II）诱导的高血压。为解析RhoBTB1逆转动脉僵硬度的潜在分子机制，本研究针对四组小鼠主动脉样本开展了批量RNA测序（bulk RNA-sequencing），四组分别为：经赋形剂或血管紧张素II处理的平滑肌特异性Cre表达小鼠（对照组），以及经赋形剂或血管紧张素II处理的平滑肌特异性RhoBTB1转基因小鼠。实验整体设计为：对分别表达平滑肌特异性Cre或平滑肌特异性RhoBTB1转基因的小鼠，经赋形剂或血管紧张素II处理后，检测其主动脉的mRNA表达谱。