Transcriptomic analysis of dynamic dysregulation in brainstem autonomic nuclei during the development of hypertension

Neurogenic hypertension stems from an imbalance in autonomic function that shifts the central cardiovascular control circuits towards a state of dysfunction. Using the female spontaneously hypertensive rat (SHR) and the normotensive Wistar Kyoto (WKY) rat model, we compared the transcriptomic changes in three autonomic nuclei in the brainstem, the nucleus of the solitary tract (NTS), caudal ventrolateral medulla (CVLM), and rostral ventrolateral medulla (RVLM) in a time series at 8, 10, 12, 16, and 24 weeks of age, spanning the pre-hypertensive stage through extended chronic hypertension. Overall design: Transcriptomic timecourse for the hypertensive SHR and normotensive WKY rats in 3 brainstem regions. Samples were collected from the nucleus of the solitary tract (NTS), rostral ventrolateral medulla (RVLM), and caudal ventrolateral medulla (CVLM) at 8, 10, 12, 16, and 24 weeks of age. N=3-6 for each sample group.

神经源性高血压（neurogenic hypertension）源于自主神经功能失衡，可导致中枢心血管调控环路陷入功能异常状态。本研究以雌性自发性高血压大鼠（spontaneously hypertensive rat, SHR）与正常血压Wistar Kyoto（WKY）大鼠为模型，针对脑干内3处自主神经核团——孤束核（nucleus of the solitary tract, NTS）、延髓尾侧腹外侧区（caudal ventrolateral medulla, CVLM）及延髓头端腹外侧区（rostral ventrolateral medulla, RVLM），在8、10、12、16及24周龄时开展时间序列转录组学分析，覆盖从高血压前期至进展性慢性高血压的完整病程。整体实验设计：本研究针对高血压模型SHR与正常血压WKY大鼠的3个脑干区域开展转录组时间进程分析。分别于8、10、12、16及24周龄采集孤束核、延髓头端腹外侧区与延髓尾侧腹外侧区样本，每个样本组的样本量为3~6。