Analysis of transgenerational effects on DNA copy number aberrations in male mice exposed to continuous 20mGy/day gamma-rays for 400 days (Secondary screening for 20mGyT familly).

Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. C57BL/6JNrs male mice were maintained in non-irradiated room for 400 days from 8 weeks of age. This study investigated the De novo mutation by comparing the parents and children. This is secondary screening. This study was performed under contract with the Aomori Prefectural Government, Japan. Two-condition experiment, C57 BL/6JNrs tail tissue non-irradiated male (20mGyTM), female (20mGyTF) and their progenies (20mGyT1 and 20mGyT2) vs. reference male mouse.

持续低剂量率（low dose-rate, LDR）γ射线照射的跨代效应尚未得到充分研究。本研究以8周龄起即于无辐照环境中饲养400天的C57BL/6JNrs雄性小鼠为实验对象，通过比较亲代与子代个体探究新生突变（de novo mutation）的发生情况。本次实验属于二次筛选，系在与日本青森县县政府签订合作合约的框架下开展。本实验采用双组对照设计：以来自尾部组织的未辐照C57 BL/6JNrs雄性小鼠（20mGyTM）、雌性小鼠（20mGyTF）及其子代（20mGyT1与20mGyT2）为实验组，以参照雄性小鼠作为对照。