Table_2_Decoding immune-related gene-signatures in colorectal neoplasia.xlsx

BackgroundColorectal cancer (CRC) is a significant health issue, with notable incidence rates in Norway. The immune response plays a dual role in CRC, offering both protective effects and promoting tumor growth. This research aims to provide a detailed screening of immune-related genes and identify specific genes in CRC and adenomatous polyps within the Norwegian population, potentially serving as detection biomarkers.
MethodsThe study involved 69 patients (228 biopsies) undergoing colonoscopy, divided into CRC, adenomatous polyps, and control groups. We examined the expression of 579 immune genes through nCounter analysis emphasizing differential expression in tumor versus adjacent non-tumorous tissue and performed quantitative reverse transcription polymerase chain reaction (RT-qPCR) across patient categories.
ResultsKey findings include the elevated expression of CXCL1, CXCL2, IL1B, IL6, CXCL8 (IL8), PTGS2, and SPP1 in CRC tissues. Additionally, CXCL1, CXCL2, IL6, CXCL8, and PTGS2 showed significant expression changes in adenomatous polyps, suggesting their early involvement in carcinogenesis.
ConclusionsThis study uncovers a distinctive immunological signature in colorectal neoplasia among Norwegians, highlighting CXCL1, CXCL2, IL1B, IL6, CXCL8, PTGS2, and SPP1 as potential CRC biomarkers. These findings warrant further research to confirm their role and explore their utility in non-invasive screening strategies.

研究背景：结直肠癌（Colorectal cancer, CRC）是一类重大公共卫生问题，在挪威发病率颇高。免疫应答在结直肠癌中发挥双重作用，既可发挥保护效应，亦可促进肿瘤生长。本研究旨在针对挪威人群的结直肠癌及腺瘤性息肉患者，开展免疫相关基因的系统性筛选并鉴定特异性基因，有望作为检测用生物标志物。
研究方法：本研究纳入69例接受结肠镜检查的患者，共获取228份活检样本，将其分为结直肠癌组、腺瘤性息肉组及对照组。通过nCounter分析检测579个免疫基因的表达水平，重点分析肿瘤组织与邻近非肿瘤组织间的差异表达情况，并针对不同患者分组开展定量逆转录聚合酶链反应（quantitative reverse transcription polymerase chain reaction, RT-qPCR）。
研究结果：核心研究发现包括：结直肠癌组织中CXCL1、CXCL2、IL1B、IL6、CXCL8（IL8）、PTGS2及SPP1的表达显著上调。此外，腺瘤性息肉组织中CXCL1、CXCL2、IL6、CXCL8及PTGS2的表达亦存在显著变化，提示上述基因可能早期参与癌变进程。
研究结论：本研究揭示了挪威人群结直肠肿瘤中独特的免疫特征，确定CXCL1、CXCL2、IL1B、IL6、CXCL8、PTGS2及SPP1可作为结直肠癌潜在的生物标志物。上述研究结果有待进一步研究以验证其作用机制，并探索其在非侵入性筛查策略中的应用价值。