Overdominant expression of related genes of ion homeostasis improves K+ content advantage in hybrid tobacco leaves. Overdominant expression of related genes of ion homeostasis improves K+ content advantage in hybrid tobacco leaves

Through a two-year field experiment, G70×GDH11 with strong heterosis and K326×GDH11 with weak heterosis were screened out. Transcriptome analyses revealed that 80.89% and 57.28% of the differentially expressed genes (DEGs) in the strong and weak heterosis combinations exhibited an overdominant expression pattern, respectively. The genes that up-regulated the overdominant expression in the strong heterosis hybrids were significantly enriched in the ion homeostasis. Genes involved in K+ transport (KAT1/2, GORK, AKT2 and KEA3), activity regulation complex (CBL-CIPK5/6), and vacuole (TPKs) genes were overdominant expressed in strong heterosis hybrids, which contributed to K+ homeostasis and heterosis in tobacco leaves. Overall design: 18 samples were analyzed, including 6 plant materials, each with three replicates.

本研究通过两年田间试验，筛选出具有强杂种优势的组合G70×GDH11与弱杂种优势的组合K326×GDH11。转录组分析结果显示，强、弱杂种优势组合中分别有80.89%和57.28%的差异表达基因（differentially expressed genes，DEGs）呈现超显性表达模式。强杂种优势组合中呈超显性上调表达的基因显著富集于离子稳态相关通路。参与钾离子（K+）转运的基因（KAT1/2、GORK、AKT2及KEA3）、活性调控复合体相关基因（CBL-CIPK5/6）以及液泡相关基因（TPKs）在强杂种优势组合中呈现超显性表达，该现象有助于烟草叶片维持钾离子稳态并形成杂种优势。实验整体设计：共分析18份样本，涵盖6种植物材料，每种材料设置3次生物学重复。