coculture SAEC and HMVEC exposed to MWCNT

This study compared global multi-walled carbon nanotube (MWCNT)-induced miRNA expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with miRNA expression from mouse lungs exposed to MWCNT. The concordant miRNA in human cell lines, mouse lung tissues and blood will be potential biomarkers for occupational and medical surveillance. Global miRNA expression profiles in monoculture and coculture HMVEC and SAEC cell lines are compared with the miRNA profiles in mouse lung tissues and blood samples exposed to MWCNT. The miRNAs with concordant MWCNT-induced reguation are identified.

本研究对比了单培养及共培养状态下，人类肺上皮细胞与微血管内皮细胞受多壁碳纳米管（multi-walled carbon nanotube, MWCNT）诱导的全基因组微小RNA（microRNA, miRNA）表达水平，并与暴露于MWCNT的小鼠肺组织中的miRNA表达水平进行了比较。在人类细胞系、小鼠肺组织与血液中表达一致的miRNA，有望成为职业与医学监测的潜在生物标志物。本研究对单培养与共培养状态下的人肺微血管内皮细胞（HMVEC）及小气道上皮细胞（SAEC）细胞系的全基因组miRNA表达谱，与暴露于MWCNT的小鼠肺组织及血液样本的miRNA表达谱进行了对比，并筛选出了受MWCNT诱导后表达调控一致的miRNA。