Impact of LXR activation on alternatively activated human macrophages

To investigate how LXR activation potentiates expression of Th2 cytokine-dependent genes in primary human macrophages, we pulsed macrophages with synthetic LXR ligand T0901317 then polarized cells to alternatively activated macrophages with IL-4 or IL-13. Human monocytes derived from three individual donors were differentiated into naïve macrophages with RPMI 1640 medium containing 5% AB-positive human serum for 7 days. Naive macrophages were pulsed with T0901317 (1 µM) for 3 hours, then media was aspirated and cells were polarized to alternatively activated macrophages with IL-4 (5 ng/ml) or IL-13 (10 ng/ml) for 24 hours.

为探究肝X受体（LXR）激活如何上调原代人巨噬细胞中Th2细胞因子依赖型基因的表达，我们先以合成LXR配体T0901317处理巨噬细胞，随后通过白细胞介素4（IL-4）或白细胞介素13（IL-13）将细胞极化为交替激活型巨噬细胞。
本研究使用来自3名健康个体供者的人单核细胞，经含5% AB型阳性人血清的RPMI 1640培养基培养7天，分化为初始巨噬细胞。将上述初始巨噬细胞以1 μM浓度的T0901317预处理孵育3小时后，吸弃培养基，再分别加入含5 ng/ml IL-4或10 ng/ml IL-13的培养基，将细胞极化为交替激活型巨噬细胞并继续培养24小时。