Figure 2. Bifidobacterium alters DC and Macrophage cytokine secretion.

Figure 2. Bifidobacterium alters DC and MF cytokine secretion. DCs (A-C) or MF (D-F) stimulated with EPS or UV-killed ATCC25526 (ATCC) or UMB-MBP-01 (MD), and 24 hrs later supernatants analyzed for A, D) IL-6, B, E) TNFa, and C, F) IL-10 by ELISA. Treatments compared using one-way ANOVA. * p value < 0.05; ** p value < 0.01, *** p value < 0.001, **** p value < 0.0001. UV-killed bacteria data representative of 3 separate experiments, 2-3 wells/culture condition, 2 technical replicates/well (supernatants from each well split and analyzed in duplicate), i.e. 4-6 wells per condition per experiment, 14 total wells per condition over 3 experiments. EPS data representative of 2 separate experiments, 2-3 wells/culture condition, 2 technical replicates/well (supernatants from each well split and analyzed in duplicate), i.e. 4 wells per condition per experiment, 10 total wells per condition over 2 experiments.

图2 双歧杆菌可改变树突状细胞（Dendritic Cell, DC）与巨噬细胞（Macrophage, MF）的细胞因子分泌水平。以胞外多糖（Extracellular Polysaccharide, EPS）、紫外线灭活的ATCC25526菌株（简称ATCC）或UMB-MBP-01菌株（简称MD）刺激树突状细胞（对应图A-C）或巨噬细胞（对应图D-F），于刺激24小时后收集细胞上清液，通过酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay, ELISA）分别检测：图A、D中白细胞介素6（Interleukin 6, IL-6），图B、E中肿瘤坏死因子α（Tumor Necrosis Factor α, TNF-α），以及图C、F中白细胞介素10（Interleukin 10, IL-10）的含量。各组间差异采用单因素方差分析（One-way Analysis of Variance, one-way ANOVA）进行比较，显著性标记规则为：* 表示P<0.05，** 表示P<0.01，*** 表示P<0.001，**** 表示P<0.0001。紫外线灭活菌组的实验数据源自3次独立重复实验：每个培养条件设置2~3个生物学重复孔，每孔设置2次技术重复（即每个孔的上清液均分后进行双份检测），因此单次实验中每个培养条件对应4~6个孔，3次实验累计每个培养条件共14个孔。胞外多糖组的实验数据源自2次独立重复实验：每个培养条件设置2~3个生物学重复孔，每孔设置2次技术重复（即每个孔的上清液均分后进行双份检测），因此单次实验中每个培养条件对应4个孔，2次实验累计每个培养条件共10个孔。