COS7 CHMP4B+VPS4DN
收藏Mendeley Data2024-01-31 更新2024-06-27 收录
下载链接:
https://data.caltech.edu/doi/10.22002/vb04b-1y387
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资源简介:
Tilt Series Date: 2015-02-07 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward Purification / Growth Conditions / Treatment: whole cells were grown on grids and transfected with Lipofectamine Sample Preparation: blotting
倾斜序列采集日期:2015年2月7日
数据采集者:科拉·伍德沃德(Cora Woodward)
物种/标本:非洲绿猴(Cercopithecus aethiops,African green monkey)
细胞株:COS-7 ATCC CRL-1651
倾斜序列采集参数:单轴倾斜模式,倾斜范围为(-60.0°,60.0°),步进角度1.0°,采用恒定角增量采集,电子束剂量为150.0 eV/Ų,欠焦量为-6.0 μm,放大倍数为22500×
采集软件:UCSFTomo
上传方式:流水线上传
所用数据处理软件:Raptor
合作者及分工:载体构建体由韦斯·桑德奎斯特(Wes Sundquist)提供;细胞转染、冷冻制样及成像工作由科拉·伍德沃德(Cora Woodward)在加州理工学院(Caltech)完成
纯化/培养条件与处理方式:完整细胞培养于载网之上,并采用Lipofectamine进行转染
样品制备:印迹法(blotting)
创建时间:
2024-01-31



