Strains used in this study.
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(i) xylose inducible, WT = wild-type isolate.
All strains were tested for hemolysis (sign for α-toxin production) on blood agar plates after 24 h and results are listed semi-quantitatively in four categories: -, no hemolysis; +/−, borderline; +, ++, efficient hemolysis. PVL production of different strains was determined by Western blot analysis of bacterial culture supernatants as described and shown in figures S1B, S1C. The results are listed semi-quantitatively in five categories: -, no PVL production; +/−, borderline; +, low; ++, +++, high amounts of PVL production.
(i) 木糖诱导型,WT即野生型分离菌株(wild-type isolate)。所有菌株均于血琼脂平板上培养24小时后检测溶血反应(α毒素产生的标志),结果以半定量方式分为四个等级:-代表无溶血;+/−代表边界弱阳性;+、++代表高效溶血。不同菌株的潘顿-瓦伦丁杀白细胞素(PVL)产生情况通过细菌培养上清液的蛋白质印迹法(Western blot)分析检测,具体方法如前文所述,结果如图S1B、S1C所示。结果以半定量方式分为五个等级:-代表无PVL产生;+/−代表边界弱阳性;+代表低水平产生;++、+++代表PVL高水平产生。
创建时间:
2010-01-08



