Gene expression profiling of rat endometrial cancer

Transcriptional profiling of rat EAC - comparison of non-/pre-malignant endometrium with endometrial tumors Endometrial cancer develops from the endometrium of the uterus and is the most common pelvic malignancy diagnosed in women in the western countries. Similar to all cancer diseases, endometrial cancer is a genetic disorder that results from complex patterns of genetic and epigenetic alterations involved in the malignant transformation. The genetic heterogeneity inherent in the human population, differences in the environment and life styles poses enormous difficulties when analyzing the complex patterns of genetic alterations contributing to cancer etiology. Inbred animal models constitute unique experimental genetic tools as the genetic heterogeneity and the influence of environmental factors can be readily reduced. The BDII/Han rat model is unique for spontaneous hormonal carcinogenesis since more than 90% of the female virgins spontaneously develop endometrial cancer during their life span. The possibility to perform global gene expression profiling of tumor cells would likely provide important information of the genes and pathways that are involved in EAC susceptibility and carcinogenesis. Keywords: Endometrial tumors developed in crosses with the BDII inbred rat strain (from backcrosses, NUT, and intercrosses, RUT) and Sprague Dawley curley-3 and Brown Norway Common reference design - Universal rat reference (Stratagene) hybridized to all arrays. Biological replicates and technical replicates (3) of each clone printed at random positions on the array.

大鼠子宫内膜癌（Endometrial Adenocarcinoma, EAC）转录组分析——非恶性及癌前子宫内膜与子宫内膜肿瘤的对照研究

子宫内膜癌起源于子宫子宫内膜，是西方国家女性确诊的最常见盆腔恶性肿瘤。与所有癌症类似，子宫内膜癌属于遗传性疾病，由参与恶性转化的复杂遗传与表观遗传改变模式所导致。人类群体固有的遗传异质性、环境与生活方式差异，给解析驱动癌症病因的复杂遗传改变模式带来了极大挑战。近交系动物模型是独特的实验遗传学工具，可有效降低遗传异质性与环境因素的影响。BDII/Han大鼠模型是自发性激素致癌的专属模型：超过90%的雌性未交配大鼠在其自然寿命中会自发罹患子宫内膜癌。

对肿瘤细胞开展全基因组基因表达谱分析，有望为阐明子宫内膜癌易感性与致癌过程相关的基因及通路提供重要信息。

关键词：样本为与BDII近交系大鼠株（回交群体NUT、互交群体RUT）、斯普拉格-道利curley-3（Sprague Dawley curley-3）以及棕色挪威（Brown Norway）大鼠杂交构建的子宫内膜肿瘤；所有芯片均采用通用大鼠参考样本（Stratagene公司）进行共同参考设计杂交。每个克隆设置3次生物学重复与技术重复，并随机排布于芯片阵列上。