Multiple gene co-options underlie the rapid evolution of sexually deceptive flowers in Gorteria diffusa

<strong>Study:</strong> Multiple gene co-options underlie the rapid evolution of sexually deceptive flowers in <em>Gorteria diffusa</em> <br> <strong>Authors:</strong> Roman T. Kellenberger, Udhaya Ponraj, Boris Delahaie, Róisín Fattorini, Janneke Balk, Sara Lopez-Gomollon, Karin H. Müller, Allan G. Ellis, Beverley J. Glover <br> <strong>Annotations:</strong> gzip compressed table containing Trinotate protein predictions and annotations of the Trinity transcripts from the combined <em>G. diffusa</em> Springbok / Naries reference transcriptome (deposited on NCBI TSA under accession GKEE00000000). <br> <strong>Differential expression:</strong> gzip compressed tables containing the results of the EdgeR mRNA differential expression analyses. Sheet A_Springbok_spotted_vs_unspott. contains the results of the differential expression analysis between spotted and unspotted ray florets of <em>Gorteria diffusa</em> Springbok. Sheet B_Springbok_spotted_vs_Naries contains the results of the differential expression analysis between spotted <em>G. diffusa</em> Springbok and spotted <em>G. diffusa</em> Naries ray florets. Sheet C_Intersection_A_and_B contains the intersection of the two previous tables,<em> i.e.</em> the Trinity transcripts differentially expressed both between spotted and unspotted <em>G. diffusa</em> Springbok ray florets, and between <em>G. diffusa</em> Springbok and Naries spotted ray florets. <br> <strong>ICP-OES:</strong> gzip compressed table containing sample input weights and volumes, and the results of the ICP-OES measurements of Iron, Zinc and Manganese concentrations in entire and divided <em>G. diffusa</em> spotted and unspotted ray florets. <br> <strong>EXPA MrBayes:</strong> Fasta file used for the MyBayes phylogenetic analysis containing the aligned protein sequences of the two conserved <em>expansin</em> domains from <em>Arabidopsis thaliana EXPA1-18</em> and <em>20-25,</em> <em>Helianthus annuus EXPA1, 4</em> and <em>6-11, </em>and <em>Gorteria diffusa EXPA1, 7</em> and <em>15.</em> <br> <strong>Differential expression small RNA:</strong> gzip compressed tables containing the results of the EdgeR small RNA differential expression analyses. Sheet A_aligned_to_supertranscripts contains the results of the small RNA differential expression analysis between spotted and unspotted ray florets of <em>Gorteria diffusa</em> Springbok using Trinity supertranscripts as reference. Sheet B_aligned_to_mirbase contains the results of the small RNA differential expression analysis between spotted and unspotted ray florets of <em>Gorteria diffusa</em> Springbok using the miRbase database as reference. <br> <strong>miRNAs:</strong> Fasta file containing the hairpin precursor sequences of pri-GdmiR156 and pri-GdmiR162 as well as the mature GdMIR156 and GdMIR162 sequences. <br>

<strong>研究主题</strong>：多基因共选择机制支撑<em>Gorteria diffusa</em>有性欺骗型花器官的快速演化<br><strong>作者</strong>：Roman T. Kellenberger, Udhaya Ponraj, Boris Delahaie, Róisín Fattorini, Janneke Balk, Sara Lopez-Gomollon, Karin H. Müller, Allan G. Ellis, Beverley J. Glover<br><strong>注释数据集</strong>：gzip压缩格式表格，包含Trinotate蛋白质预测结果，以及来自<em>G. diffusa</em> Springbok与Naries组合参考转录组的Trinity转录本注释信息，该参考转录组已提交至NCBI TSA，登录号为GKEE00000000。<br><strong>差异表达分析数据</strong>：gzip压缩格式表格，包含EdgeR mRNA差异表达分析结果。工作表A_Springbok_spotted_vs_unspott. 对应<em>Gorteria diffusa</em> Springbok有色斑点与无色斑点舌状花的差异表达分析结果；工作表B_Springbok_spotted_vs_Naries 对应<em>G. diffusa</em> Springbok有色斑点舌状花与<em>G. diffusa</em> Naries有色斑点舌状花的差异表达分析结果；工作表C_Intersection_A_and_B 为前两个表格的交集，即同时在<em>G. diffusa</em> Springbok有色与无色斑点舌状花之间，以及<em>G. diffusa</em> Springbok与Naries有色斑点舌状花之间差异表达的Trinity转录本。<br><strong>ICP-OES（电感耦合等离子体发射光谱，Inductively Coupled Plasma Optical Emission Spectrometry）</strong>：gzip压缩格式表格，包含样品称样量与体积，以及<em>G. diffusa</em>有色斑点与无色斑点舌状花（整朵及分切样品）的铁、锌、锰浓度ICP-OES检测结果。<br><strong>EXPA MrBayes</strong>：用于MrBayes系统发育分析的Fasta格式文件，包含比对后的蛋白质序列，序列来源包括<em>Arabidopsis thaliana</em> EXPA1-18、EXPA20-25，<em>Helianthus annuus</em> EXPA1、EXPA4、EXPA6-11，以及<em>Gorteria diffusa</em> EXPA1、EXPA7、EXPA15的两个保守expansin（膨胀素）结构域。<br><strong>小RNA差异表达分析数据</strong>：gzip压缩格式表格，包含EdgeR小RNA差异表达分析结果。工作表A_aligned_to_supertranscripts 对应以Trinity超转录本为参考序列，对<em>Gorteria diffusa</em> Springbok有色斑点与无色斑点舌状花开展的小RNA差异表达分析结果；工作表B_aligned_to_mirbase 对应以miRbase数据库为参考序列，对<em>Gorteria diffusa</em> Springbok有色斑点与无色斑点舌状花开展的小RNA差异表达分析结果。<br><strong>miRNAs（微小RNA，microRNA）</strong>：Fasta格式文件，包含pri-GdmiR156与pri-GdmiR162的茎环前体序列，以及成熟GdMIR156与GdMIR162序列。