Mass spectrometry raw data for "Proteomics reveals substantial differences between in vitro matured abattoir-derived and in vivo matured oocytes in cattle"

In vitro production (IVP) of bovine embryos still has its limitations such as low blastocyst rate and lower embryo quality, resulting in lower pregnancy rates following the transfer of IVP embryos compared to in vivo produced embryos. Given these differences in developmental competence, RNA sequencing and microarray technology have been applied to describe the differences in transcriptional activity between in vitro and in vivo produced embryos. All but one of these studies solely utilized oocytes obtained from slaughterhouse material for the in vitro production of embryos, thereby introducing the possibility, that differences between IVP and in vivo embryos are in part attributable to differing sources of oocytes. The aim of the present study was therefore to compare the proteome of oocytes retrieved from slaughterhouse material, with and without a period of in vitro maturation and in vivo matured oocytes obtained from donor cattle following superovulation. For each group the protein pattern of four biological replicates containing ten oocytes each were analyzed via SWATHTM-MS.

牛胚胎体外生产（In vitro production, IVP）仍存在诸多局限，具体表现为囊胚率偏低、胚胎质量欠佳，相较于体内生产的胚胎，移植体外生产胚胎后的妊娠率也更低。鉴于二者发育能力存在差异，已有研究借助RNA测序与基因芯片（microarray）技术，解析体外与体内生产胚胎的转录活性差异。不过上述研究中，除一项外均仅采用屠宰场获取的卵母细胞开展胚胎体外生产，这使得体外生产与体内胚胎的差异可能部分源于卵母细胞来源不同。因此本研究旨在对比三类卵母细胞的蛋白质组：分别为屠宰场获取的未经体外成熟的卵母细胞、该类卵母细胞经体外成熟培养后的样本，以及经超数排卵处理的供体牛体内成熟卵母细胞。本研究对每组设置4个生物学重复，每个重复包含10枚卵母细胞，通过SWATHTM-MS技术分析其蛋白质谱。