Demultiplexing Ig repertoires by parallel mRNA/DNA sequencing shows major differential alterations in severe COVID-19. Demultiplexing Ig repertoires by parallel mRNA/DNA sequencing shows major differential alterations in severe COVID-19

To in-depth analyze the anti-SARS-CoV-2 humoral response and find elements that can lead or prevent acute respiratory distress syndrome (ARDS), we dissected the multiple layers of B cell responses by NGS immunoglobulin repertoires on RNA template from peripheral blood cell of severe COVID-19 patients. Overall design: Blood samples from 19 Intensive Care Unit patients infected with SARS-CoV-2 were included in this study (12 with ARDS and 7 without ARDS) and were followed over multiple time points (D0, D7 and 4 months after admission). Samples from healthy volunteers (24 samples), recruited between the ages of 18 and 75 and free from any pathology were used as controls.

为深入解析抗新型冠状病毒（Severe Acute Respiratory Syndrome Coronavirus 2, SARS-CoV-2）的体液免疫应答，并筛选可诱发或预防急性呼吸窘迫综合征（Acute Respiratory Distress Syndrome, ARDS）的关键调控因子，本研究以重症COVID-19患者外周血血细胞的RNA为模板，借助下一代测序（Next-Generation Sequencing, NGS）开展免疫球蛋白组库分析，以此剖析B细胞应答的多层级特征。研究整体设计：本研究纳入19例感染SARS-CoV-2的重症监护病房（Intensive Care Unit, ICU）患者血液样本（其中12例合并ARDS，7例未合并ARDS），并在多个时间节点（入院当日D0、入院第7日D7及入院后4个月）进行连续跟踪采样；同时招募24例年龄介于18至75岁、无任何基础病理异常的健康志愿者，采集其血液样本作为对照。