Engystomops pustulosus (Tungara frog) genome, aEngPut1, sequence data

The Tungara frog (Engystomops pustulosus) is a species of frog in the family Leptodactylidae. It is a small nocturnal terrestrial frog found in Mexico, Central America, and the northeastern regions of South America. The tungara frog exhibits interesting behavior in male/female interactions. Male vocalizations are critical in female mate choice, and females often prefer males who give complex mating calls at a lower frequency rather than simple calls at a higher frequency (from Wikipedia entry). This sample of a female individual (aEngPut1/MGP-1488.1) was used to sequence Hi-C scaffolding for a trio assembly (offspring aEngPut4/MGP-1488.2, mother aEngPut2/MGP-1444.1, father aEngPut3/MGP-1452.1). The individuals were bred in captivity and procured by Marcos Gridi-Papp to generate a high-quality reference genome for the Vertebrate Genomes Project (VGP). Sequencing and genome assembly were conducted at the Vertebrate Genome Lab (VGL) at the Rockefeller University, led by Erich D. Jarvis, Giulio Formenti, and Jennifer Balacco.

泡蟾（Tungara frog，学名*Engystomops pustulosus*）是细趾蟾科（Leptodactylidae）下的蛙类物种。该物种为小型夜行性陆栖蛙类，分布于墨西哥、中美洲及南美洲东北部区域。泡蟾的雌雄个体互动行为颇具研究价值：雄性鸣叫是雌性择偶的关键影响因素，雌性通常更偏好以较低频率发出复杂求偶鸣唱的雄性，而非以较高频率发出简单鸣唱的雄性（资料来源：维基百科词条）。本研究中的雌性个体样本（aEngPut1/MGP-1488.1）被用于三联体组装（trio assembly）的Hi-C支架构建，该三联体组装包含子代个体aEngPut4/MGP-1488.2、母本aEngPut2/MGP-1444.1及父本aEngPut3/MGP-1452.1。这些个体均为人工繁育所得，由Marcos Gridi-Papp为脊椎动物基因组计划（Vertebrate Genomes Project, VGP）获取，用于构建高质量参考基因组。测序及基因组组装工作在洛克菲勒大学脊椎动物基因组实验室（Vertebrate Genome Lab, VGL）开展，该项目由Erich D. Jarvis、Giulio Formenti及Jennifer Balacco主导。