DRG-CSC co-culture tissue engineered model, CSCs monoculture model and DRG monoculture model. DRG-CSC co-culture tissue engineered model, CSCs monoculture model and DRG monoculture model

In this study, we constructed a tissue-engineered DRG-CSC (corneal stromal cells) co-cultured model (DS model), a CSC monoculture model (S model), and a DRG monoculture model (D model). Chicken DRGs were extracted from E7-E10 chicken embryo. Human CSCs were extracted in stromal lenticules from small incision lenticule extraction (SMILE). Transcriptional profiling of DRG in the DS model (DS-D), DRG in the D model(D-D), CSCs in the DS model (DS-S), CSCs in the S model (S-S) was analyzed. After 7 days of culture, RNA of each group was extracted. Overall design: DS-D vs D-D, DS-S vs S-S. There are three biological replicates in each group.

本研究构建了组织工程化背根神经节（Dorsal Root Ganglion, DRG）-角膜基质细胞（corneal stromal cells, CSC）共培养模型（简称DS模型）、CSC单培养模型（简称S模型）以及DRG单培养模型（简称D模型）。鸡DRG提取自E7至E10期鸡胚；人CSC从小切口基质透镜取出术（small incision lenticule extraction, SMILE）获取的角膜基质透镜中分离获得。本研究对DS模型中的DRG（记为DS-D）、D模型中的DRG（记为D-D）、DS模型中的CSC（记为DS-S）以及S模型中的CSC（记为S-S）的转录组谱进行了分析。培养7天后，提取各组样本的RNA。实验设计包含两组对照比较：DS-D与D-D、DS-S与S-S；每组均设置3次生物学重复。