Deep mutational scanning of avian influenza PB2 to identify host-adaptive mutations

This study aims to comprehensively map the effect of mutations to an avian influenza PB2 protein that enhance growth in different host cells. We mutagenized the PB2 gene from the influenza strain A/Green-winged Teal/ Ohio/175/1986, and used these mutant plasmid libraries to generate mutant virus libraries, which we then passaged in human and avian cells. We deep sequenced the mutant plasmid and virus libraries along with unmutated plasmid and virus controls.

本研究旨在全面解析禽流感PB2蛋白（avian influenza PB2 protein）的突变对不同宿主细胞内病毒增殖能力的影响。研究团队对流感毒株A/Green-winged Teal/Ohio/175/1986的PB2基因开展诱变操作，利用构建得到的突变体质粒文库生成突变病毒文库，随后在人类细胞与禽源细胞中对该病毒文库进行传代培养。同时，研究团队对突变体质粒文库、突变病毒文库，以及未突变质粒与病毒对照样本进行了深度测序。