CIL:20151

Adapted from the original movie of a Balamuthia mandrillaris within a culture of monkey kidney cells (CIL:20152). The comparatively thick body of the ameba has a number of pseudopodia extending from it and a large prominent nucleus. The action of the pseudopodia is important in the feeding of the amebas. Their tips have a pointed, sharp, dense extension that has been seen to grow out, and move together with a snap (about 1 second), an action that is shown in the 2X enlargement and pointed out by the circles that have been added. This action is a typical movement of the tips of the pseudopodia. Accompanying photographs are enlargements of the tips of pseudopodia to demonstrate their flexibility and movement, and a second set of photographs that demonstrate the puncture of the plasma membrane of a rounded, clear monkey kidney cell with a large, rounded, clear nucleus with an elongated dense nucleolus. The first shows an indentation in the cell membrane, the withdrawal of the pseudopodia tip and the subsequent rupture of the membrane in the area indented by the amebic tip. The eventual break in the membrane may have resulted from the release of a proteolytic enzyme. Adapted with permission. Details of preparation can be found to accompany CIL:19325.

本内容改编自一株曼德里拉巴拉姆希阿米巴（Balamuthia mandrillaris）在猴肾细胞（CIL:20152）培养体系中的原始影像。该阿米巴相对肥厚的虫体上伸出多根伪足（pseudopodia），并带有一个大而显著的细胞核。伪足的运动对于阿米巴的摄食至关重要。其顶端带有尖锐致密的突起，可向外伸出并伴随一次时长约1秒的快速弹缩运动，该过程在2倍放大画面中得以呈现，并由添加的圆圈标注。该动作是伪足顶端的典型运动模式。随附的影像包含两组：第一组为伪足顶端的放大画面，用以展示其柔韧性与运动状态；第二组影像则展示了一株形态圆润透亮的猴肾细胞，其带有大而圆润透亮的细胞核及细长致密的核仁（nucleolus），该细胞的质膜（plasma membrane）被穿刺的完整过程。第一组影像展示了细胞膜被阿米巴顶端压陷、伪足顶端回缩，以及随后在阿米巴顶端压陷区域发生的细胞膜破裂过程。最终的细胞膜破裂可能是由蛋白水解酶（proteolytic enzyme）的释放所引发的。本内容经授权改编。具体制备细节可参考CIL:19325的配套资料。