Sequencing SARS-CoV-2 from Antigen Cards

This project demonstrates that it is possible to produce SARS-CoV-2 whole genome sequences from antigen test-derived anterior nares swabs. Viral RNA was extracted from BinaxNOW COVID-19 Ag Card (Abbott Diagnostics Scarborough) swabs and SARS-CoV-2 whole genome sequence libraries were prepared using an amplicon-based NGS panel (Paragon Genomics: CleanPlex SARS-CoV-2 FLEX Panel). Prepared libraries were then sequenced on the MiSeq system at 2 x 151 bp using the MiSeq v3, (600 cycle) kit (Illumina, Cat. MS-102-3003).

本研究证实，可从抗原检测所得的前鼻拭子中获取严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）全基因组序列。研究人员从BinaxNOW COVID-19抗原检测卡（雅培诊断斯卡伯勒分部，Abbott Diagnostics Scarborough）配套拭子中提取病毒RNA，并采用基于扩增子的下一代测序（Next-Generation Sequencing, NGS）试剂盒（Paragon Genomics公司：CleanPlex SARS-CoV-2 FLEX试剂盒）构建SARS-CoV-2全基因组测序文库。制备完成的文库随后于MiSeq测序系统上进行双端151碱基对测序，所用试剂为MiSeq V3（600循环）测序试剂盒（Illumina公司，货号：MS-102-3003）。