RNA sequencing of murine bone marrow dendritic cells and monocytes following TLR1/2 stimulation. RNA sequencing of murine bone marrow dendritic cells and monocytes following TLR1/2 stimulation

Treatment with a TLR1/2 agonist induces expression of interleukin-1beta that regulates hematopoietic stem cell number and function Overall design: Wildtype mice were treated with a single dose of the TLR1/2 agonist PAM3CSK4 and then dendritic cells and monocytes were purified from the bone marrow for RNA sequencing

采用Toll样受体1/2激动剂（TLR1/2 agonist）处理可诱导白细胞介素-1β（interleukin-1beta）的表达，进而调控造血干细胞（hematopoietic stem cell）的数量与功能。实验设计：对野生型小鼠单次给予Toll样受体1/2激动剂PAM3CSK4处理，随后从骨髓中纯化树突状细胞与单核细胞，用于RNA测序（RNA sequencing）。