Examples of carbohydrases and CBM proteins detected in S. degradans supernatants by mass spectrometry.
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1Protein was detected in supernatants of cultures grown in the following growth substrates: Avicel (��70% crystalline cellulose), xylan (Birchwood glucuronoarabinoxylan).2Predictions of function and module determination utilizing the routines used for the updates of the CAZy database (www.cazy.org/CAZY/).3Module abbreviations: CBM, carbohydrate binding module; UNK, unknown function; PSL, polyserine linker; LPB, lipobox signature sequence; GH, glycosyl hydrolase; EPR, glutamate-proline rich region.4Gene number having the prefix ��Sde��, for Saccharophagus degradans as assigned in Jun 15, 2005 genome assembly5MW and amino acid count calculated using the protParam tool at http://us.expasy.org/tools/ based on DOE/JGI gene model amino acid sequence translations including any predicted signal peptide.
1 在以如下生长底物培养的培养液上清中检测到蛋白质:Avicel(含≥70%结晶纤维素)、木聚糖(Birchwood glucuronoarabinoxylan,桦木糖醛酸阿拉伯木聚糖)。
2 功能预测与模块注释采用了用于更新CAZy数据库(www.cazy.org/CAZY/)的标准分析流程。
3 模块缩写说明:CBM(carbohydrate binding module,碳水化合物结合模块)、UNK(unknown function,未知功能)、PSL(polyserine linker,聚丝氨酸接头)、LPB(lipobox signature sequence,脂盒特征序列)、GH(glycosyl hydrolase,糖苷水解酶)、EPR(glutamate-proline rich region,富谷氨酸-脯氨酸区域)。
4 以"Sde"为前缀的基因编号,对应2005年6月15日发布的基因组组装版本中注释的Saccharophagus degradans(糖噬降解单胞菌)。
5 分子量(MW)与氨基酸残基总数基于DOE/JGI基因模型的氨基酸序列翻译结果(包含所有预测的信号肽),通过http://us.expasy.org/tools/ 站点的protParam工具计算得到。
创建时间:
2015-12-02



