Epigenetic silencing of noncoding RNA nc886 provokes oncogenes during esophageal tumorigenesis. Homo sapiens

nc886 is a 101 nucleotides long non-coding RNA that is also known as a precursor microRNA or a vault RNA. nc886 has been suggested to be a tumor suppressor, mainly inferred by its expression pattern as well as its genomic location at human chromosome 5q31, a locus for a tumor suppressor gene(s). Overall design: Pre-clinical study In order to understand potential roles of nc886 in esophageal squamous cell carcinoma (ESCC), we performed the knockdown of nc886 in nonmalignant HET-1A cells and ESCC cell lines (TE-1 and TE-8) and identified and analyzed genes whose expression is activated by nc886 knockdown in ESCC.

nc886是一种长度为101个核苷酸的非编码RNA（non-coding RNA），亦被称为前体微RNA（precursor microRNA）或穹窿RNA（vault RNA）。nc886已被提示为一种肿瘤抑制因子，该结论主要基于其表达模式，以及它在人类5号染色体q31区域的基因组定位——该区域正是肿瘤抑制基因的所在位点。整体实验设计：本研究为临床前研究。为阐明nc886在食管鳞状细胞癌（esophageal squamous cell carcinoma, ESCC）中的潜在作用，我们分别在非恶性HET-1A细胞与ESCC细胞系（TE-1、TE-8）中对nc886进行敲低，并鉴定并分析ESCC中经nc886敲低后表达激活的基因。