Data_Sheet_1_Toward a Consensus in the Repertoire of Hemocytes Identified in Drosophila.pdf

The catalog of the Drosophila immune cells was until recently limited to three major cell types, based on morphology, function and few molecular markers. Three recent single cell studies highlight the presence of several subgroups, revealing a large diversity in the molecular signature of the larval immune cells. Since these studies rely on somewhat different experimental and analytical approaches, we here compare the datasets and identify eight common, robust subgroups associated to distinct functions such as proliferation, immune response, phagocytosis or secretion. Similar comparative analyses with datasets from different stages and tissues disclose the presence of larval immune cells resembling embryonic hemocyte progenitors and the expression of specific properties in larval immune cells associated with peripheral tissues.

此前，基于形态学、功能及少量分子标记，果蝇（Drosophila）免疫细胞的分类目录仅局限于三种主要细胞类型。近期三项单细胞（single cell）研究揭示了果蝇幼虫免疫细胞存在多个亚群，展现出其分子特征的高度多样性。由于上述三项研究采用了略有差异的实验与分析方法，本研究对这些数据集进行了比较分析，最终鉴定出8个共有的、稳健的亚群，这些亚群与增殖、免疫应答、吞噬作用或分泌等不同功能相关。通过对不同发育阶段及组织来源的数据集开展同类比较分析，本研究还发现了一类类似胚胎血细胞祖细胞（hemocyte progenitors）的幼虫免疫细胞，以及与外周组织相关的幼虫免疫细胞所特有的属性表达特征。