Gene expression profile at single cell level of CD11b+/CD45+ myeloid cells from the brain (scRNA_male). Gene expression profile at single cell level of CD11b+/CD45+ myeloid cells from the brain (scRNA_male)

We used single cell RNA sequencing (scRNA-seq) to analyze the subsets of myeloid cells affected by deletion of miR-155 in microglia. Overall design: CD11b+/CD45+ myeloid cells were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using scRNAseq.

我们采用单细胞RNA测序（single cell RNA sequencing, scRNA-seq），分析了小胶质细胞中微小RNA-155（miR-155）敲除所影响的髓系细胞亚群。实验整体设计：通过荧光激活细胞分选术（Fluorescence-activated cell sorting, FACS）分离获得CD11b阳性/CD45阳性髓系细胞，并运用scRNA-seq完成测序分析。