Transcriptional analyses of the Erythrobacter litoralis DSM 8509 general stress response system

We measured steady-state transcript levels in 1) wild-type E. litoralis DSM 8509, 2) a nepR-ecfG deletion strain (ΔnepR-ecfG), 3) a phyR deletion strain (ΔphyR), 4) a lovK-lovR deletion strain (ΔlovKR), and 5) a gsrK-gsrP deletion strain (ΔgsrKΔgsrP) cultivated in constant white light or in constant dark conditions. Cultures of Erythrobacter litoralis DSM 8509 wild-type and mutant strains were grown in 0.5x marine broth in the presence or absence of white light.

本研究检测了以下菌株的稳态转录本水平，所有菌株均在0.5倍浓度海水肉汤中培养，并分别置于持续白光照射或持续黑暗环境下：1）野生型海滨赤杆菌（Erythrobacter litoralis）DSM 8509；2）nepR-ecfG基因缺失菌株（ΔnepR-ecfG）；3）phyR基因缺失菌株（ΔphyR）；4）lovK-lovR基因缺失菌株（ΔlovKR）；5）gsrK-gsrP基因缺失菌株（ΔgsrKΔgsrP）。