Growth Induction of Cavendish Buds (musa Acuminata L.) on Difference Concentration IBA and BA In Vitro

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.

卡文迪什香蕉（Musa acuminata L.）的传统育苗方式存在生长周期较长、质量不均一的问题，且可能携带源自不健康母株的病害。本研究旨在探究不同浓度的吲哚丁酸（Indole-3-butyric acid, IBA）与6-苄氨基嘌呤（6-Benzylaminopurine, BA）对组培苗生长的影响，并确定体外培养条件下促进卡文迪什香蕉芽组培苗生长的IBA与BA最优浓度组合。实验于2018年4月至7月在印度尼西亚西爪哇省苏梅当县Pasir Banteng园艺种子发展研究所组织培养实验室开展。研究采用完全随机设计（Completely Randomized Design），设置两个因素：第一因素为IBA浓度，包括0 mg/L（i0）、0.75 mg/L（i1）和1.5 mg/L（i2）三个水平；第二因素为BA浓度，包括1 mg/L（b1）、3 mg/L（b2）、5 mg/L（b3）和7 mg/L（b4）四个水平，每个处理重复三次。差异显著性检验采用邓肯多重范围检验（Duncan Multiple Range Test, DMRT）。结果显示，培养6周后（6 weeks after culture, WAC），IBA与BA浓度在芽生长和叶片数指标上存在交互作用；IBA浓度对芽数指标具有独立影响；1.5 mg/L IBA与3 mg/L BA的组合为促进卡文迪什香蕉芽外植体生长的最优浓度。