Infection of MK2 cells with monkeypox virus. Macaca mulatta

Orthopox viruses, including monkeypox, multiply intracellularly and induce numerous changes in host genes expression. The virus target mainly humoral host response, and simultaneously, exploits other genes and functions to reproduce effectively. The goal of this experiment is to identify those host genes and functions that are essential for monkeypox virus replication. Overall design: Mock infected control cells were treated and incubated identically to time point arms, except for virus exposure. Two time points of cells infected with monkeypox virus were harvested at 3, 7 hours post infection, and gene expression was assessed using microarray in all arms. The experiment was done in triplicate.

正痘病毒（Orthopox viruses），包括猴痘病毒（monkeypox），可在宿主细胞内增殖，并诱导宿主基因表达发生诸多显著改变。该病毒主要靶向宿主体液免疫应答，同时劫持宿主其他基因及相关功能以实现高效增殖。本实验旨在鉴定出对猴痘病毒复制至关重要的宿主基因与功能。整体实验设计：未感染病毒的空白对照细胞，其处理与培养流程与各时间点实验组完全一致，唯一区别为未接触病毒。本实验在感染后3小时、7小时两个时间点收集猴痘病毒感染的细胞样本，并通过基因芯片（microarray）对所有组别开展基因表达水平检测。本实验设置三次生物学重复。