Transcriptomic profiling of brassinosteroids response genes in soybean (Glycine max L.)

Brassinosteroids (BRs) are a series of sterol hormones in plants, which play important physiological roles in many aspects of plant growth and development processes. Exogenous application of BR and its inhibitors PPZ to investigated the roles of BR in soybean root growth and development. Transcriptome sequencing analysis was performed to identified lots number of differential expression genes(DEGs) by eBL (2,4-epicastasterone, 24-epiCS) and PPZ (propiconazole) treatment of soybean root tips(1 cm). Overall design: Soybean seeds were germinated in vermiculite for 3 days. 3-day-old soybean seedlings were transferred to hydroponics medium for 5 days, then with 0.1 µM PPZ and mock treatment separately for another 2.5 days. 3-day-old soybean seedlings were transferred to hydroponics medium for 7 days, then treated with 0.01 nM eBL and mock for another half a day. PPZ_BR treatment is that 5-day-old hydroponic cultured soybean seedlings treat with 0.1 µM PPZ for 2 days and then with 0.01 nM BL for half a day.

油菜素甾醇类激素（Brassinosteroids, BRs）是一类植物甾醇类激素，在植物生长发育的诸多方面发挥重要生理功能。本研究通过外施BR及其抑制剂PPZ，探究BR在大豆根系生长发育中的作用；并对经eBL（2,4-表油菜素甾酮，24-epiCS）与PPZ（丙环唑，propiconazole）处理的1 cm大豆根尖开展转录组测序分析，鉴定得到大量差异表达基因（differential expression genes, DEGs）。
实验整体设计如下：
1. 大豆种子在蛭石中萌发3天，将3日龄大豆幼苗转移至水培培养基中培养5天，随后分别用0.1 μM PPZ及空白对照处理2.5天；
2. 将3日龄大豆幼苗转移至水培培养基中培养7天，随后用0.01 nM eBL及空白对照处理半天；
3. PPZ-BR联合处理组：将培养5天的水培大豆幼苗先用0.1 μM PPZ处理2天，再用0.01 nM BL处理半天。