Impact of RNA isolation methods for RNA-seq on gene expression. Here RNA was isolated from human peripheral blood mono nuclear cells (PBMCs) using either Columns or magnetic beads and the isolated RNA was processed using prime-seq

Here silica column and bead based RNA isolation was performed. The two methods were compared using prime-seq to determine the effect of the RNA isolation method on gene expression quantification. This was tested for three different types of input samples, PBMCs, HEK293T cells and striatal mouse tissue to compare the RNA isolation over a range of inputs. For each sample and experimental condition 8 technical replicates were performed.

本研究采用基于硅胶柱与磁珠的两种方法开展RNA提取。通过Prime-seq技术对这两种方法进行比较，以探究不同RNA提取方案对基因表达定量分析的影响。我们选取三类不同的起始样本：外周血单个核细胞（PBMCs）、HEK293T细胞以及小鼠纹状体组织，在不同起始样本量范围内对比两种RNA提取方法的应用效果。针对每一类样本与实验条件，均设置8次技术重复。