Bacillus licheniformis Transcriptome or Gene expression

Bacillus licheniformis is widely used for the large-scale industrial production of proteins. We identified the strong promoters for gene expression by dissecting the transcriptome of B. licheniformis ATCC14580 in post-log phase. In total, 3,959 genes of 4,208 B. licheniformis genes were transcribed under the selected growth condition. Transcriptional start sites (TSS) of 197 annotated genes and 1249 operons were identified. To screen for strong promoters, the candidate promoter pBL9 displayed the strongest beta-galactosidase specific activity, which suggests that it could be used effectively for heterologous gene expression.

地衣芽孢杆菌（Bacillus licheniformis）被广泛应用于蛋白质的大规模工业化生产。本研究通过解析处于对数生长期后期的地衣芽孢杆菌ATCC14580的转录组，筛选并鉴定了可用于基因表达的强启动子。在所选定的培养条件下，该菌株的4208个基因中共有3959个基因发生转录。本研究共鉴定得到197个已注释基因与1249个操纵子的转录起始位点（Transcriptional Start Sites, TSS）。在筛选强启动子的过程中，候选启动子pBL9展现出最高的β-半乳糖苷酶（beta-galactosidase）比活性，表明该启动子可有效应用于异源基因表达。