TMT-based proteomic analysis of CSF in dogs with intervertebral disc herniation

In our study, MS-based high-resolution proteomic analysis of CSF have been employed in order to gain deeper insights into changes occurring during IVDH in dogs, monitoring the pathophysiological processes at the protein and metabolite levels. The proteomics study involved canine patients classified within two groups. Healthy dogs (control group) (N=6) were mix breeds (20 kg average body weight), age 2-6 years, while the intervertebral disc herniation group (N=9) were mixed breeds (body weight 6-15 kg), age 7-12 years. In IVDH dogs, disc herniation was detected in T13/L1/L2 discs by CT. The dogs in IVDH group were paraplegic with preserved deep pain reflex and underwent a surgery procedure for decompression of the spinal cord.

本研究采用基于质谱（Mass Spectrometry, MS）的脑脊液（Cerebrospinal Fluid, CSF）高分辨率蛋白质组学分析方法，以深入解析犬椎间盘突出（Intervertebral Disc Herniation, IVDH）病程中的分子变化，从蛋白质和代谢物层面监测其病理生理过程。本蛋白质组学研究将受试犬分为两组：健康对照组（N=6）为混血品种，平均体重20 kg，年龄2~6岁；椎间盘突出组（N=9）同样为混血品种，体重6~15 kg，年龄7~12岁。椎间盘突出组受试犬经CT检查确认存在T13/L1/L2节段的椎间盘突出，均表现为截瘫但保留深部痛反射，并接受了脊髓减压手术治疗。