Positionally distinct interferon-stimulated dermal immune-acting fibroblasts promote neutrophil recruitment in Sweet's syndrome [snRNA-seq]

Sweet's syndrome (SS) is a poorly understood inflammatory skin disease. Using RNA sequencing from patients with SS, Pyoderma_Gangrenosum (PG) and healthy controls, in the study, the gaps in the understanding of cellular and molecular landscape of SS and PG skin are addressed. Overall design: Human skin biopsies from affected individuals were collected and processed for library prep and next-generation sequencing at the Advanced Genomics Core at the University of Michigan.

Sweet综合征（Sweet's syndrome, SS）是一种尚未被充分阐明的炎症性皮肤病。本研究针对SS、坏疽性脓皮病（Pyoderma Gangrenosum, PG）患者及健康对照者开展RNA测序（RNA sequencing），填补了学界对SS与PG皮肤病变的细胞及分子特征图谱的认知空白。实验设计：收集受累患者的皮肤活检组织，于密歇根大学先进基因组学核心实验室完成文库制备（library prep）与下一代测序（next-generation sequencing）。