Runx3 function in CD4+ splenic dendritic cells

CD4+ dendritic cells are part of the innate immunity essential for priming and activating of CD4+ T cells To identify Runx3 responsive genes CD4+ dendritic cells were sorted from freshly isolated macs enriched splenic DCs taken from 6 weeks old mice. Six samples from six mice were sorted and analyzed where in each littermates pair consisted of a control and Runx3 KO.

CD4+树突状细胞（CD4+ dendritic cells）是固有免疫的重要组成部分，其对启动并激活CD4+ T细胞至关重要。为鉴定Runx3响应基因，研究人员从6周龄小鼠体内新鲜分离、经磁活化细胞分选（MACs）富集的脾脏树突状细胞（splenic DCs）中分选得到CD4+树突状细胞。本实验共对6只小鼠的6份样本完成分选与分析，每对同窝仔鼠分别设置野生型对照组与Runx3基因敲除（Runx3 KO）组。