Additional file 3 of Prunus persica plant endogenous peptides PpPep1 and PpPep2 cause PTI-like transcriptome reprogramming in peach and enhance resistance to Xanthomonas arboricola pv. pruni

Additional file 3. Interactive principal component analysis (PCA) of regularized–logarithm normalized expression data from the 500 genes showing the highest variance (DESeq2 1.20.0 package [60]) conducted in R [63] using scatter plot 3d library [73]. Three principal components, PC1, PC2 and PC3, with Eigenvalues above 1 explained 83.84%, 7.04% and 2.81% of the overall variability, respectively. The peptide treatments are shown in different colors: tan, time zero (t0); turquoise, 1 h PpPep1 (P1t1); light green, 1 h PpPep2 (P2t1); blue, 24 h PpPep1 (P1t24); pink, 24 h PpPep2 (P2t24); orange, 48 h PpPep1 (P1t48); yellow, 48 h PpPep2 (P2t48). Three biological replicates per sample are shown.

附加文件3：针对方差排名前500的基因经正则化对数归一化后的表达数据，借助R语言[63]中的scatter plot 3d库[73]，结合DESeq2 1.20.0软件包[60]完成的交互式主成分分析（Principal Component Analysis，简称PCA）。特征值大于1的PC1、PC2、PC3三个主成分分别解释了总变异的83.84%、7.04%与2.81%。不同颜色对应不同肽处理组别：棕褐色为0小时对照组（t0）；湖蓝色为1小时 PpPep1 处理组（P1t1）；淡绿色为1小时 PpPep2 处理组（P2t1）；蓝色为24小时 PpPep1 处理组（P1t24）；粉色为24小时 PpPep2 处理组（P2t24）；橙色为48小时 PpPep1 处理组（P1t48）；黄色为48小时 PpPep2 处理组（P2t48）。每个样本均设置3次生物学重复并予以展示。