Expression stability of the reference genes calculated by BestKeeper software.

Notes: SR, roots exposed to high-salt treatment; SL, leaves exposed to high-salt treatment; PR, roots exposed to PEG treatment; PL, leaves exposed to PEG treatment; HL, leaves exposed to heat treatment; CL, leaves exposed to cold treatment. Descriptive statistics of 10 candidate genes based on the coefficient of variance (CV) and standard deviation (SD) of their Cq values were determined using the whole data set. Reference genes were identified as the most stable genes, i.e. those with the lowest coefficient of variance and standard deviation (CV ± SD).

备注：SR指经高盐处理的根系（roots exposed to high-salt treatment）；SL指经高盐处理的叶片（leaves exposed to high-salt treatment）；PR指经聚乙二醇（PEG）处理的根系（roots exposed to PEG treatment）；PL指经聚乙二醇（PEG）处理的叶片（leaves exposed to PEG treatment）；HL指经热胁迫处理的叶片（leaves exposed to heat treatment）；CL指经冷胁迫处理的叶片（leaves exposed to cold treatment）。本研究基于10个候选基因的Cq值的变异系数（coefficient of variance, CV）与标准差（standard deviation, SD），通过全数据集计算其描述性统计量。内参基因被鉴定为表达稳定性最优的基因，即变异系数与标准差（CV ± SD）均最低的基因。