Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 11/04/2004 to 11/08/2004 (Dumont d'Urville - Hobart)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 11/04/2004 to 11/08/2004 on a trip from Dumont D'Urville to Hobart.

Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).

Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

这些数据由“阿斯特罗实验室号”考察船（RV L'Astrolabe，平台代码：FHZI）于2004年4月11日至2004年8月11日期间，在从杜蒙·迪尔维尔（Dumont D'Urville）前往霍巴特（Hobart）的航行中采集。

光系统II（PSII）的最大光化学效率，又称PSII的最大量子产率（Fv/Fm），已成为浮游植物研究中应用最广泛的荧光参数之一。它代表潜在光化学效率，即光合机构捕获的光能被用作光化学反应的概率。研究表明，Fv/Fm对理化性质的变化具有即时响应，可作为浮游植物整体健康状况或生理活性的诊断指标。结合PSII的吸收截面积和叶绿素浓度，该参数可用于测量初级生产力（Cheah等，2011，《深海研究》）。

3米深度的海水通过船上的清洁海水管道持续供应。使用由100个亚饱和闪光（闪光持续时间1.1微秒，闪光间隔2.8微秒）和20个弛豫闪光（闪光持续时间1.1微秒，闪光间隔51.6微秒）组成的闪光序列，在暗适应状态下（≥15分钟暗适应）以1分钟为间隔持续测量快速重复率（FRR）荧光产量。