Repression of germline genes in C. elegans somatic tissues by H3K9 dimethylation of their promoters [ChIP-seq]. Repression of germline genes in C. elegans somatic tissues by H3K9 dimethylation of their promoters [ChIP-seq]

synMuv B proteins, including LIN-15B, are necessary to repress the expression of germline-promoting genes in the soma during embryonic and early larval development in C. elegans. The goal of this research was to determine if changes to histone modifications could underlie the misexpression of germline genes in these mutants. We performed ChIP-seq on four histone modifications in wild type and three synMuv B mutants at two temperatures to assess these changes. Overall design: Examination of four histone modifications (H3K9me2, H3K27me3, H3K4me3, H3K36me3) by ChIP in WT and three mutant (lin-15B(n744), lin-35(n745), lin-37(n758)) C. elegans at 20°C and 26°C to assess changes in chromatin that may lead to the changes in gene expression seen in mutants.

synMuv B蛋白（synMuv B proteins）包括LIN-15B，在秀丽隐杆线虫（C. elegans）的胚胎发育与早期幼虫阶段，其对体细胞内生殖系促进基因的表达抑制是必需的。本研究旨在探究组蛋白修饰的改变是否为此类突变体中生殖系基因异常表达的潜在诱因。我们在两种温度条件下，针对野生型（wild type，WT）与三种synMuv B突变体的四种组蛋白修饰开展染色质免疫共沉淀测序（ChIP-seq），以评估相关变化。整体实验设计：于20℃及26℃温度条件下，采用染色质免疫共沉淀（ChIP）技术，对野生型秀丽隐杆线虫（C. elegans）及三种突变体（lin-15B(n744)、lin-35(n745)、lin-37(n758)）的四种组蛋白修饰（H3K9me2、H3K27me3、H3K4me3、H3K36me3）进行检测，以评估可能导致突变体出现基因表达改变的染色质变化。