Endometrial cell-type specific RNA-seq

Cell-type specific RNA-seq is a powerful approach for unravelling molecular processes of endometrial receptivity, and to detect novel sensitive biomarkers of receptivity. Overall design: 16 paired endometrial tissue samples from pre-receptive (defined as LH2) and receptive phase endometria (defined as LH8) from Estonia (defined as E) and Spain (defined as S) were collected. CD9-positive epithelial cells (defined as epithelium) and CD13-positive stromal cells (defined as stroma) were isolated with fluorescent activated cell sorting (FACS) and full transcriptome analysis was performed by RNA-seq.

细胞类型特异性RNA测序（cell-type specific RNA-seq）是解析子宫内膜容受性分子进程、发掘新型容受性敏感生物标志物的有力研究手段。整体实验设计：本研究共收集16对配对子宫内膜组织样本，样本供体分别来自爱沙尼亚（记为E）与西班牙（记为S），涵盖着床前窗口期（定义为LH2）与容受窗口期（定义为LH8）两个时期的子宫内膜组织。通过荧光激活细胞分选（fluorescent activated cell sorting, FACS）分离得到CD9阳性上皮细胞（定义为上皮组分）与CD13阳性基质细胞（定义为基质组分），随后采用RNA测序完成全转录组分析。