Dysregulation of different classes of tRNA fragments in chronic lymphocytic leukemia

We analyzed small RNA sequencing data from CD5+/CD19+ B cells of a cohort of indolent and aggressive CLL patients compared with CD19+ B-cells of healthy donors. We identified tsRNA signatures in indolent and aggressive CLL vs. normal B-cells; we also found a drastic dysregulation of the expression of mature tRFs in CLL. A total of 20 whole blood samples provided by the CLL Research Consortium and grouped into the following two categories: 10 indolent CLL and 10 aggressive CLL. A third category of 8 CD19+ B-cell control samples (courtesy of J. Byrd MD, IRB approved protocol) was also considered in the study.

本研究针对惰性与侵袭性慢性淋巴细胞白血病（Chronic Lymphocytic Leukemia, CLL）患者队列的CD5+/CD19+ B细胞的小RNA测序数据展开分析，并以健康供者的CD19+ B细胞作为对照。研究人员在惰性与侵袭性CLL样本中分别鉴定出与正常B细胞存在差异的转运RNA衍生小RNA (transfer RNA-derived small RNAs, tsRNA) 特征谱，同时发现CLL样本中成熟tRNA衍生片段 (tRNA-derived fragments, tRFs) 的表达存在显著失调。本研究共纳入由CLL研究联盟提供的20份全血样本，分为以下两组：10份惰性CLL样本与10份侵袭性CLL样本。此外，本研究还纳入了由J. Byrd医学博士惠赠的8份CD19+ B细胞对照样本，该组样本的获取使用了经伦理审查委员会 (Institutional Review Board, IRB) 批准的研究方案。