Gene expression changes in Apc-mutant mouse intestinal organoids with and without deleting the Prox1 transcription factor

We isolated and selected intestinal adenoma organoids from villin-CreER; Apcflox/flox and villin-CreER; Apcflox/flox; Prox1flox/flox mice and added tamoxifen to induce the deletion of the Apc and Prox1 genes in the intestinal epitheliul ex vivo. Microarray experiments were carried out 7 days after the addition of tamoxifen. Total RNA obtained from villin-CreER; Apcflox/flox and villin-CreER; Apcflox/flox; Prox1flox/flox organoids were compared 7 days after the addition of tamoxifen and 5 days after the selection for Apc-mutant organoids in the absence of the Wnt-agonist R-Spondin1.

本研究从villin-CreER; Apcflox/flox及villin-CreER; Apcflox/flox; Prox1flox/flox基因工程小鼠中分离并筛选肠腺瘤类器官（intestinal adenoma organoids），通过添加他莫昔芬（tamoxifen）在体外诱导肠上皮内Apc与Prox1基因的敲除。于添加他莫昔芬7天后开展基因芯片（Microarray）实验。分别于添加他莫昔芬后7天，以及在不含Wnt激动剂R-脊椎蛋白1（R-Spondin1）的培养体系中完成Apc突变型类器官筛选后的第5天，对上述两类基因型类器官所提取的总RNA进行比较分析。