Analysis of effects of TM4SF5 deficiency in inguinal white adipose tissue. Analysis of effects of TM4SF5 deficiency in inguinal white adipose tissue

There are limited studies of TM4SF5, even though it plays a role in cell proliferation functions as lysosomal membrane amino acid sensor. In current studies, we aimed to study the role of TM4SF5 in mitochondrial homeostasis in adipocytes. Overall design: White adipose tissue mRNA profiles of wild type(WT) and TM4SF5-/- mice were generated by deep sequencing. Three biological replicates were analyzed for each of two conditions.

尽管跨膜4超家族成员5（TM4SF5）作为溶酶体膜氨基酸感受器参与调控细胞增殖过程，但目前针对该蛋白的相关研究仍较为匮乏。本研究旨在探究TM4SF5在脂肪细胞线粒体稳态中的调控作用。实验整体设计：通过深度测序获取野生型（WT）及TM4SF5纯合基因敲除小鼠的白色脂肪组织mRNA表达谱；两种实验分组各设置3个生物学重复样本进行测序分析。