ATAC-seq in hippocampal GABAergic cells of SMARCA3 cKO mice

Purpose: To identify chromatin accessibility changes in CCK hippocampal interneurons from SMARCA3 cKO mice Method: Genetically expressing GFP nuclei were sorted from CCK+ or GAD2+ cells from WT or SMARCA3 cKO mice, and were used for ATAC seq using Nextseq sequencer. Results: Biostatistical analysis identified 3552 genes that were altered by SMARCA3 cKO in CCK cells. Overall design: 6 hippocampi were pooled for one ATAC sample. We used mice that were CCK cre+/GAD2 cre+ crossed with L10-EGFP+ mice and SMARCA3 fl/fl and littermates without SMARCA3 fl/fl controls.

【研究目的】鉴定SMARCA3条件性敲除（SMARCA3 cKO）小鼠海马胆囊收缩素（Cholecystokinin, CCK）阳性中间神经元的染色质可及性变化。【实验方法】从野生型（Wild Type, WT）或SMARCA3条件性敲除（SMARCA3 cKO）小鼠的CCK阳性或谷氨酸脱羧酶2（Glutamic Acid Decarboxylase 2, GAD2）阳性细胞中分选表达绿色荧光蛋白（Green Fluorescent Protein, GFP）的细胞核，采用Nextseq测序仪进行ATAC测序（ATAC-seq）。【实验结果】经生物统计学分析，在CCK阳性细胞中共有3552个基因的表达水平因SMARCA3条件性敲除而发生改变。【实验设计】将6个海马组织混合制备一个ATAC测序样本；实验所用小鼠为CCK Cre+/GAD2 Cre+小鼠分别与L10-EGFP+小鼠、SMARCA3 flox/flox（SMARCA3 fl/fl）小鼠杂交获得的子代，同时以不携带SMARCA3 fl/fl的同窝小鼠作为对照。