Microbial communities and bacterial indicators for shoreline sand, sediment, and water in Racine, Wisconsin; Chicago, Illinois; and East Chicago, Indiana; 2016-2017

The data associated with the following data release were collected between 2016 and 2017 at three locations on Lake Michigan: Racine, WI; Chicago, IL; and East Chicago, IN. Individual water samples were collected one day a week for ten weeks between June and August. Samples were collected from eight specific sites made up of two river and six shoreline type environments. Sampling was completed at sites where various morphology (embayment, sand and sediment characteristics, size and shape) and hydrologic conditions (currents and waves) were present. Then samples were analyzed using microbial communities (metagenomic analysis), markers of contamination (microbial source tracking), and fecal indicator bacteria (E. coli).

本次发布数据集相关的数据采集工作于2016年至2017年间开展，采集地点为密歇根湖（Lake Michigan）的三处区域：威斯康星州拉辛市（Racine, WI）、伊利诺伊州芝加哥市（Chicago, IL）以及印第安纳州东芝加哥市（East Chicago, IN）。研究人员于2016年6月至8月期间，每周采样一次，连续开展十周，每次获取单份水体样本。本次采样共设置8个特定采样点，涵盖2种河流型环境与6种岸线型环境。采样工作覆盖所有具备多样地貌特征（含凹岸形态、沙质与沉积物特性、空间尺度与形状）及水文条件（水流与波浪）的采样点位。随后对采集的样本开展三类分析：针对微生物群落的宏基因组分析（metagenomic analysis）、污染标志物检测（微生物源追踪技术，microbial source tracking），以及粪便指示菌（大肠杆菌，E. coli）检测。